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Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column

Separation of highly hydrophili...
We published "Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column" in MethodsX, 2023, 10. 102061.


Highly hydrophilic compounds such as nicotinamide metabolites are very difficult to separate via high-performance liquid chromatography (HPLC) using octadecyl (C18) columns. In general, for the separation of hydrophilic compounds, hydrophilic interaction liquid chromatography (HILIC) columns are used instead of reversed phase chromatography using C18 columns. However, HILIC columns generally obey complex separation mechanisms because ionic interactions are involved in the retention process, which hinders the optimization of the separation conditions. Additionally, the resulting peak shapes are disturbed when large amounts of aqueous samples are injected. This study demonstrates that COSMOSIL PBr columns, in which both hydrophobic and dispersive interactions occur, show high retention for various hydrophilic compounds under similar separation conditions as those used with C18 columns. Specifically, using a COSMOSIL PBr column, 11 nicotinamide metabolites could be separated under simpler conditions than those used previously with C18 columns, affording better peak shape for each compound. The applicability of the method was evaluated using a tomato sample, from which the nicotinamide metabolites were successfully separated. The results show that the COSMOSIL PBr column is a good alternative to the C18 column for a good separation of all the peaks, including impurity peaks.

The following products were used in this study.
COSMOSIL PBr Packed Column
#NMN #hilic #hplc #lcms #nicotinamide #reference

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Press Release: Separation of amyloid β fragment peptides using DL-Amino Acid Labeling Kit

Press Release: ...
#Aminoacid #Chiral #amiroid #drug #hplc #peptide #pressrelease

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Separation of amyloid β fragment peptides with racemised and isomerised aspartic acid residues using an original chiral resolution labeling reagent

Separation of a...
We are pleased to announce that the study using our DL-amino acid labeling kit, made in collaboration with Professor Hideaki Kakeya from Graduate School of Pharmaceutical Sciences, Kyoto University, was selected for inclusion in HOT Articles 2023 by the Royal Society of Chemistry journal Analyst, 2023, Advance Article

The following products were used in this study.
DL-Amino Acid Labeling Kit
#Aminoacid #DL #HPLC #Labeling #Reference #chiral

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