Nacalai USA's Blog

Lineup June 2023

**20%-off Special Promotion for IHC products!**

<Promo Code>
Blocking One Hist: BOH23JUN
https://www.nacalaiusa.com/products/view/193/blocking-reagent-blocking-one-histo

HistoVT One: HV23JUN
https://www.nacalaiusa.com/products/view/111/antigen-retrieval-solution-histovt-one

Signal Enhancer HIKARI for Immunostain: HI23JUN
https://www.nacalaiusa.com/products/view/110/antibody-diluent-signal-enhancer-hikari-for-immunostain

BCIP-NBT Solution: BN23JUN
https://www.nacalaiusa.com/products/view/501/bcip-nbt-solution-ready-to-use

Fluoro-KEEPER: FK23JUN
https://www.nacalaiusa.com/products/view/258/antifade-reagent-fluoro-keeper-non-hardening-type

**30%-off CosmoPAGE precast gel & WB ladder**

<Promo Code>
CosmoPAGE precast gels: CP2023SP
https://www.nacalaiusa.com/products/view/398/cosmopage-precast-gel

CosmoPAGE WB Ladder: WBL23JUN
https://www.nacalaiusa.com/products/view/515/cosmopage-wb-ladder

We are attending the AACR Annual Meeting 2023 in Orland, FL.
Please stop by our booth No. 2542.
We will be available from 9:00 am on Apr 18 to 12:30 pm on Apr 19. 
There will also be a campaign exclusively for AACR participants!!!

We are pleased to release a new version of our COSMOSIL catalog, which includes our new RNA and oligonucleotide column series.

COSMOSIL/COSMOCORE HPLC Catalog 12th Edition

We exhibited at #PITTCON 2023 at Pennsylvania Convention Center from March 20-22.

We are running a special promotion of SunShell, SunArmor, Sunniest and Sunrise columns. For a limited period of time, all columns can be purchased at a 20% off the list price using the promo code SUN20.

SunShell HPLC columns
SunShell SFC columns
SunArmor HPLC columns
Sunniest HPLC columns
Sunrise HPLC columns

The discount will be:
- applied only to the columns listed on our website.
- not available to overseas.
- cannot be combined with any other offer, promotion or discount.

*** PromoCode: SUN20 ***
*** Valid until 04/30/2023 ***

For users of flow cytometers, this is a mesh-equipped microplate into which specimens can be dispensed using a multichannel pipette, an automatic dispenser, or other tools. The microplate can be stacked on top of another one, and then the specimens can be filtered at once by centrifugation. This is a convenient solution for those who want to operate more easily and save time.

30%-off CosmoPAGE Precast Gel.

*End Date: 4/30/2023

Please click the link below for more information:

CosmoPAGE Precast Gel

20%-off  Protease Inhibitor Cocktail Tablets, EDTA-Free (Cat#: NU01069, 20 tablets) and RIPA Buffer (10X) (Cat#: 08714-04, 1 set).
Please use the promo code "PICT20" for your purchase.

*End Date: 3/31/2023

Please click the link below for more information:

Protease Inhibitor Cocktail Tablets, EDTA-Free

RIPA Buffer

Lineup March 2023
Small Molecules/Cytokines for Stem Cell Research, Protease Inhibitor Cocktail
Tablets, EDTA-Free, etc.

We published "Separation of highly hydrophilic nicotinamide metabolites using a COSMOSIL PBr column" in MethodsX, 2023, 10. 102061.

DOI: https://doi.org/10.1016/j.mex.2023.102061

Abstract: 
Highly hydrophilic compounds such as nicotinamide metabolites are very difficult to separate via high-performance liquid chromatography (HPLC) using octadecyl (C18) columns. In general, for the separation of hydrophilic compounds, hydrophilic interaction liquid chromatography (HILIC) columns are used instead of reversed phase chromatography using C18 columns. However, HILIC columns generally obey complex separation mechanisms because ionic interactions are involved in the retention process, which hinders the optimization of the separation conditions. Additionally, the resulting peak shapes are disturbed when large amounts of aqueous samples are injected. This study demonstrates that COSMOSIL PBr columns, in which both hydrophobic and dispersive interactions occur, show high retention for various hydrophilic compounds under similar separation conditions as those used with C18 columns. Specifically, using a COSMOSIL PBr column, 11 nicotinamide metabolites could be separated under simpler conditions than those used previously with C18 columns, affording better peak shape for each compound. The applicability of the method was evaluated using a tomato sample, from which the nicotinamide metabolites were successfully separated. The results show that the COSMOSIL PBr column is a good alternative to the C18 column for a good separation of all the peaks, including impurity peaks.